Projects | Ayomi Ratnayake, M.S.

This project investigates the contribution of PMCA2 to mechanisms underlying the processing of nociceptive and pain signals in the spinal cord dorsal horn by use of genetically modified mice, tissue culture and animal models of injury and disease. Read More
This project investigates the effects of TLR9 and TLR7 ligands on locomotor, pain and bladder dysfunction in mice sustaining a contusion injury.  The TLR ligands are delivered intrathecally.  Injury is induced by use of the Infinite Horizon Imapctor.  Locomotor function is assessed by open field... Read More
This project defines stimuli that can promote the beneficial effects and attenuate the detrimental outcomes of inflammation in spinal cord injury and in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis.  Molecular and cellular approaches including flow cytometry,... Read More
This project focuses on the mechanisms underlying plasma membrane calcium ATPase and collapsing response mediator protein-mediated neuronal degeneration and neuroprotection in experimental autoimmune encephalomyelitis and spinal cord injury.  A combination of in vivo models, spinal cord neuronal... Read More
This project investigates the cross-talk between spinal cord neurons and glia in the context of TLR activation or inhibition, using purified neuron-glia co-cultures. Read More
This project investigates the behavioral and molecular alterations resulting from the genetic deletion of TLR9 during development.  TLR9 knockout mice are used as a model.  The behavioral paradigms assess motor function, sensory reactivity and learning.   Read More
The goal of this project is to determine whether maternal immune stimulation during pregnancy influences the vulnerability of the offspring to experimental autoimmune encephalomyelitis during adulthood.  Read More
State-of-the art proteomic approaches are used to unravel disease mechanisms and define new targets for therapeutic interventions. Read More